CF071 - Métodos e Modelos Experimentais para Estudo do Câncer - 2S/2026 Imprimir

Ementa:

A disciplina abordará os principais métodos experimentais, in vitro, in vivo e translacionais, para estudo do câncer, considerando os aspectos gerais das células tumorais (cancer hallmarks): migração, proliferação, sobrevivência/morte, ciclo celular, microambiente tumoral (angiogênese e evasão da resposta imune). A abordagem será teórica, prática e demonstrativa.

Bibliografia:

ABDOLAHI, S. et al. Patient-derived xenograft (PDX) models, applications and challenges in cancer research. Journal of Translational Medicine, v. 20, 2022.
ARNAOUTOVA, I.; KLEINMAN, H. K. In vitro angiogenesis: endothelial cell tube formation assay. Nature Protocols, v. 5, n. 4, p. 628–635, 2010.
ARORA, S. et al. Spheroids in cancer research: recent advances and future perspectives. Biomedicine & Pharmacotherapy, v. 174, 2024.
BINNEWIES, M. et al. Understanding the tumor immune microenvironment (TIME) for effective therapy. Nature Medicine, v. 24, p. 541–550, 2018.
COSSARIZZA, A. et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, v. 51, p. 2708–3145, 2021.
DE VLEESCHAUWER, S. I. et al. OBSERVE: guidelines for the refinement of rodent cancer models. Nature Protocols, 2024.
FRANKEN, N. A. P. et al. Clonogenic assay of cells in vitro. Nature Protocols, v. 1, n. 5, p. 2315–2319, 2006.
HANAHAN, D. Hallmarks of cancer: new dimensions. Cancer Discovery, v. 12, n. 1, p. 31–46, 2022.
HETTIARACHCHI, P. et al. Choice of animal models to investigate cell migration and metastasis in cancer. Cancers, v. 17, 2776, 2025.
IRESON, C. R. et al. The role of mouse tumour models in the discovery and development of anticancer drugs. British Journal of Cancer, v. 121, p. 101–108, 2019.
JIN, J. et al. Challenges and prospects of patient-derived xenografts for cancer research. International Journal of Molecular Sciences, v. 24, 2023.
JUSTUS, C. R. et al. In vitro cell migration and invasion assays. Journal of Visualized Experiments (JoVE), n. 88, e51046, 2014.
LIVAK, K. J.; SCHMITTGEN, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, v. 25, n. 4, p. 402–408, 2001.
POZAROWSKI, P.; DARZYNKIEWICZ, Z. Analysis of cell cycle by flow cytometry. Methods in Molecular Biology, v. 281, p. 301–311, 2004.
RISS, T. L. et al. Cell viability assays. In: SITTAMPALAM, G. S. et al. (Ed.). Assay Guidance Manual. Bethesda: Eli Lilly & Company and the National Center for Advancing Translational Sciences, 2016.
STRIBBLING, S. M. et al. Orthotopic and metastatic tumour models in preclinical cancer research. Advanced Drug Delivery Reviews, v. 214, 115019, 2024.

Ano de Catálogo: 2026

Créditos: 4